MST4/MST3/STK25 [p Thr190, p Thr174, p Thr178] Antibody (EP2123Y)

Product Discontinued

MST4/MST3/STK25 [p Thr190, p Thr174, p Thr178] Antibody (EP2123Y) Summary

• Immunogen: A phospho specific peptide corresponding to residues surrounding threonine 178 of human MST4, threonine 190 of human MST3 and threonine 174 of human STK25.
• Modification: p Thr190, p Thr174, p Thr178
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STK26
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Western Blot 1:1000-10000
• Application Notes: This product is useful for: Western Blot, Immunocytochemistry. In Western blot this antibody detects a band at approximately 52kDa.

Reactivity Notes

Reacts with: Human Does not react with Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for MST4/MST3/STK25 [p Thr190, p Thr174, p Thr178] Antibody (EP2123Y)

• Mammalian STE20-like protein kinase 4
• MASK
• Mst3 and SOK1-related kinase
• MST4 serine/threonine protein kinase MST4
• MST-4
• Serine/threonine-protein kinase MASK
• Serine/threonine-protein kinase MST4
• STE20-like kinase MST4

Background

Novel human Ste20-related kinase Mst4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation (1). Wild type and C-terminally truncated forms of Mst4 can both induce apoptosis upon overexpression in mammalian cells that is abrogated by CrmA, suggesting involvement of Mst4 in the apoptotic machinery in mammalian cells (2). Mst4 was found to be expressed in prostate carcinoma tumor samples and cell lines. In addition, expression levels appeared to correlate with tumorigenicity and androgen receptor status of the cells. Mst4 kinase activity was stimulated significantly by epidermal growth factor receptor ligands, which are known to promote growth of prostate cancer cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MST4/MST3/STK25 Antibody (NB100-80004)(1)

Publication using NB100-80004

• Cornils H, Kohler RS, Hergovich A et al. Human NDR kinases control G(1)/S cell cycle transition by directly regulating p21 stability. Mol Cell Biol 2011-04-01 [PMID: 21262772]

Bioinformatics

• Gene Symbol: STK26
• Entrez:
  • 51765(Human)
• Uniprot:
  • Q9P289(Human)