MRPL3 Antibody

Western Blot: MRPL3 Antibody [NB300-863] (1ug/ml) of HepG2 cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MRPL3 Antibody Summary

• Immunogen: Peptide with sequence NVCQPGAPSITFA corresponding to C-Terminus according to NP_009139.1.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MRPL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in HepG2 lysates (calculated MW of 38.6 kDa band according to NP_009139.1).
• Control:

  MRPL3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRPL3 Antibody

• 39S ribosomal protein L3, mitochondrial
• mitochondrial ribosomal protein L3
• MRL3mitochondrial, L3
• RPML3

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRPL3 Antibody (NB300-863)(1)

Publication using NB300-863

• Ou JH, Yen TS, Wang YF et al. Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells. Nucleic Acids Res 1987-11-11 [PMID: 2891103]

Bioinformatics

• Gene Symbol: MRPL3
• Entrez:
  • 11222(Human)
• Uniprot:
  • NP_009139.(Human)