MPP7 Overexpression Lysate

Images

 
Western Blot: MPP7 Overexpression Lysate (Adult Normal) [NBL1-13206] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MPP7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MPP7 Overexpression Lysate Summary

Description

MPP7 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206096

Accession#: NM_173496

Protein Tag: C-MYC/DDK
Gene
MPP7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
65.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MPP7 Overexpression Lysate

  • FLJ32798
  • MAGUK p55 subfamily member 7
  • membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)

Background

Membrane-associated guanylate kinases (MAGUKs) are important adaptor proteins involved in the assembly of protein complexes at sites of cell-cell contact. They are found in synapses, adherens junctions, and tight junctions. All MAGUKs contain at least 1 PDZ domain, an SH3 domain, and a GUK domain, and many contain 1 or 2 L27 domains, which are involved in multimerization of MAGUKs. MPP7 belongs to the p55 stardust subfamily of MAGUKs, which is named for a Drosophila gene required for establishment of cell polarity in the developing fly embryo (Bohl et al., 2007 [PubMed 17237226]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-14193
Species: Hu
Applications: IHC, IHC-P
NBP2-99481
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NB600-1229
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IP, WB
H00004354-M01
Species: Hu
Applications: ELISA, ICC/IF, IP, S-ELISA, WB
NB300-952
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-19388
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-81185
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-87839
Species: Hu
Applications: IHC, IHC-P
NB100-1607
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IP, WB
NBP2-94240
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89679
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-87365
Species: Hu
Applications: IHC, IHC-P, WB
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NB100-1354
Species: Hu
Applications: Flow, IF, PEP-ELISA
DHAPG0
Species: Hu
Applications: ELISA
AF5550
Species: Mu, Rt
Applications: WB
NBP1-84671
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-00594
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
1719-SE
Species: Hu
Applications: EnzAct
NBP2-94064
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for MPP7 Lysate (NBL1-13206) (0)

There are no publications for MPP7 Lysate (NBL1-13206).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MPP7 Lysate (NBL1-13206) (0)

There are no reviews for MPP7 Lysate (NBL1-13206). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MPP7 Lysate (NBL1-13206) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MPP7 Products

Bioinformatics Tool for MPP7 Lysate (NBL1-13206)

Discover related pathways, diseases and genes to MPP7 Lysate (NBL1-13206). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MPP7 Lysate (NBL1-13206)

Discover more about diseases related to MPP7 Lysate (NBL1-13206).
 

Pathways for MPP7 Lysate (NBL1-13206)

View related products by pathway.

PTMs for MPP7 Lysate (NBL1-13206)

Learn more about PTMs related to MPP7 Lysate (NBL1-13206).
 

Research Areas for MPP7 Lysate (NBL1-13206)

Find related products by research area.

Blogs on MPP7

There are no specific blogs for MPP7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MPP7 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol MPP7