MOBKL2B Antibody (OTI6C6)

Western Blot: MOBKL2B Antibody (6C6) [NBP2-02843] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MOBKL2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MOBKL2B.

Immunocytochemistry/Immunofluorescence: MOBKL2B Antibody (6C6) [NBP2-02843] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MOBKL2B.

Flow Cytometry: MOBKL2B Antibody (6C6) [NBP2-02843] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MOBKL2B antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI6C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.68 mg/ml

MOBKL2B Antibody (OTI6C6) Summary

• Immunogen: Full length human recombinant protein of human MOBKL2B (NP_079037) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MOB3B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 25.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.68 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MOBKL2B Antibody (OTI6C6)

• Em:AL163192.1
• FLJ13204
• FLJ23916
• Mob1 homolog 2b
• MOB1, Mps One Binder kinase activator-like 2B (yeast)
• MOB3BMGC32960
• monopolar spindle 1 binding, MOB1, domain containing
• mps one binder kinase activator-like 2B
• Protein Mob3B

Background

The protein encoded by this gene shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MOB3B
• Entrez:
  • 78917(Human)