MID2 Antibody

Western Blot: MID2 Antibody [NB100-1167] - Analysis of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35ug protein in RIPA buffer).

Immunohistochemistry-Paraffin: MID2 Antibody [NB100-1167] - Analysis of Human Kidney using this antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MID2 Antibody Summary

• Description: Novus Biologicals Goat MID2 Antibody (NB100-1167) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-MID2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence QESPYVSGMKTCH corresponding to C-Terminus according to NP_036348.2, NP_438112.2.
• Localization: Cytoplasmic
• Specificity: This antibody is expected to recognise both human isoforms of this protein.
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MID2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 80-85 kDa band observed in mouse and rat heart, and rat kidney lysates (calculated MW of 77.8 kDa band according to mouse NP_035975.1 and 83.1 kDa band according to rat NP_001178818.1). IHC-P: Human kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules.
• Control:

  MID2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MID2 Antibody

• EC 6.3.2.-
• FLJ37715
• FLJ41813
• FXY2midin-2
• midin 2
• Midin-2
• midline 2
• Midline defect 2
• Midline-2
• RING finger protein 60
• RNF60midline-2
• TRIM1probable E3 ubiquitin-protein ligase MID2
• tripartite motif protein 1
• Tripartite motif-containing protein 1

Background

MID2 is a member of the tripartite motif (TRIM) family. The MID motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of the gene encoding MID2 results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MID2 Antibody (NB100-1167)(1)

Publication using NB100-1167

• Buchner G, Montini E, Andolfi G et al. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet 1999-08-01 [PMID: 10400986]

Bioinformatics

• Gene Symbol: MID2
• Entrez:
  • 11043(Human)
  • 23947(Mouse)
• Uniprot:
  • NP_036348(Human)