METTL14 Antibody

Western Blot: METTL14 Antibody [NBP2-76359] - Samples: Whole cell lysate (15 ug) from Jurkat, RKO, GaMG, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METTL14 antibody NBP2-76359 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

METTL14 Antibody [NBP2-76359] - Detection of human METTL14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METTL14 antibody NBP2-76359 used for IP at 100 ul per reaction. METTL14 was also immunoprecipitated by rabbit anti-METTL14 antibody NBP2-76819. For blotting immunoprecipitated METTL14, NBP2-76359 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.

Product Discontinued

METTL14 Antibody Summary

• Immunogen: Immunogen sequence between amino acids 25 and 75
• Predicted Species: Mouse (100%), Bovine (100%), Orangutan (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: METTL14
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 50-100 ul/mg lysate
  • Western Blot 1:1000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Tris-buffered Saline containing 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for METTL14 Antibody

• EC 2.1.1
• EC 2.1.1.-
• KIAA1627
• KIAA1627methyltransferase-like protein 14
• methyltransferase like 14
• METTL14

Background

METTL14 forms a heterodimer with METTL3, creating an N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METTL14