Recombinant Human METTL1 His Protein

SDS-Page: Recombinant Human METTL1 His Protein [NBP1-50953] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human METTL1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-276 of Human METTL1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK NFPAIFRRIQ DPVLQAVTSQ TSLPGH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: METTL1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT, 50 mM NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human METTL1 His Protein

• C12orf1
• D1075-like gene product
• EC 2.1.1.33
• FLJ95748
• methyltransferase like 1
• methyltransferase-like 1
• Methyltransferase-like protein 1
• TRM8
• tRNA (guanine-N(7)-)-methyltransferase
• tRNA(m7G46)-methyltransferase
• YDL201w

Background

METTL1 is known as tRNA (guanine-N(7)-)-methyltransferase which belongs to the methyltransferase superfamily. METTL1 shows high sequence similarity to yeast ORF YDL201w and has been shown to be inactivated by phosphorylation. This protein contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphylation. It catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Recombinant human METTL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: METTL1