Human Melanoma Tissue MicroArray (Malignant)

Product Discontinued

Human Melanoma Tissue MicroArray (Malignant) Summary

• Description: Microarray Panel:
  Malignant melanoma
  No. of samples: 59
  No. of patients: 59
  Core diameter: 2.0 mm
  Format: Formalin Fixed
  Section thickness: 4 micrometer
  Please see manual for tissue information and location.
  
  All tissues are fixed in 10% neutral buffered formalin for 12 to 24 hours, dehydrated with gradient ethanol, cleared with xylene, and embedded in paraffin. Then each slide is tested for immunohistochemistry on multiple antibodies including the p53 protein. Quality control is ensured as every tissue block is collected and arranged by certified pathologists, who used an identical process for their own research.
  
  About 100,000 cells are included in each sample with 2 mm core diameter. Some slides may have less than 60 cores due to sample loss when obtaining multiple sections. Maximum number of missing cores in a slide is less than 10%.
• Gene: CDKN2A

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Details for Array

• Type: Tissue
• Tissue Condition: Malignant

Alternate Names for Human Melanoma Tissue MicroArray (Malignant)

• ARF
• CDK4 inhibitor p16-INK4
• CDK4IP14ARF
• CDKN2
• cell cycle negative regulator beta
• CMM2P16-INK4A
• Cyclin-dependent kinase 4 inhibitor A
• cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• cyclin-dependent kinase inhibitor 2A
• INK4
• INK4A
• MLMP16INK4
• MTS-1
• MTS1P14
• Multiple tumor suppressor 1
• p14
• p14ARF
• P16
• p16-INK4
• P16INK4A
• p16-INK4a
• P19
• P19ARF
• TP16

Background

Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Tissue Micro Arrays are guaranteed for 1 year from date of receipt.

Publications for Melanoma Tissue Micro Array (NBP2-30285)(7)

Publications using NBP2-30285

• Zhang W, Zhang C, Li W et al. CD8(+) T-cell immunosurveillance constrains lymphoid pre-metastatic myeloid cell accumulation. Eur. J. Immunol. 2014-10-14 [PMID: 25310972] (IHC-P, Human)
• Das SK, Bhutia SK, Sokhi UK et al. Raf kinase inhibitor RKIP inhibits MDA-9/syntenin-mediated metastasis in melanoma. Cancer Res. 2012-10-11 [PMID: 23066033] (IHC-P)
• Laga AC, Zhan Q, Weishaupt C et al. SOX2 and nestin expression in human melanoma: an immunohistochemical and experimental study. Exp Dermatol. 2011-04-01 [PMID: 21410764] (IHC-P)
  
  Details:
  IHC (paraffin): Labeled with SOX2 and Nestin antibodies, Table 1 & Fig S1.
• Kapoor A, Goldberg MS, Cumberland LK et al. The histone variant macroH2A suppresses melanoma progression through regulation of CDK8. Nature. 2010-12-23 [PMID: 21179167] (IHC-P)
  
  Details:
  IHC (paraffin): Labeled with antibodies to H2A2, H3, and other H2A variants, Fig 1B.
• Bair WB 3rd, Cabello CM, Uchida K et al. GLO1 overexpression in human malignant melanoma. Melanoma Res. 2010-04-01 [PMID: 20093988] (IHC-P)
  
  Details:
  IHC (paraffin) & labelled with a GLO1 antibody (Fig 2): 1. Malignant Melanoma: IMH-3692. Skin Cancer & Normal Skin: IMH-323.
• Laga AC, Lai CY, Zhan Q et al. Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology. Am J Pathol. 2010-02-01 [PMID: 20042675] (IHC-P)
  
  Details:
  IHC (paraffin): Labeled with SOX2 antibody, results described on page 908.
• Baldi A, Lombardi D, Russo P et al. Ferritin contributes to melanoma progression by modulating cell growth and sensitivity to oxidative stress. Clin Cancer Res. 2005-05-01 [PMID: 15867210] (IHC-P)
  
  Details:
  IHC (paraffin): Data described in results on page 3180.

FAQs for Melanoma Tissue Micro Array (NBP2-30285). (Showing 1 - 1 of 1 FAQs).

1. We are interested in the Human Melanoma Tissue Microarray slide (NBP2-30285). We need to do IHC for some phospho- and methyl-proteins, so will probably need fresh-cut slides. Are these prepared from fresh tissues and suitable for such analyses? Do you also provide IHC and pathology analysis services?
   • Here are some specifications and application details: Large cores of 2.0 mm in diameter. Fresh-cut slides. Compatible with automatic IHC/ISH staining system (Ventana, Leica, Dako, etc.). Individual slide is put in an air-tight pack with inert gas. Tissue array slides are shipped at room temperature. If the slides are stored in 4 degree Celsius , they are good up to one year. The slides are useful for IHC/FISH/mRNA in situ hybridization/miRNA in situ hybridization/TUNEL for apoptosis & Nucleic acid extraction so they should be valuable for the IHC you want to perform. I am afraid we do not provide IHC and Pathology.

Bioinformatics

• Gene Symbol: CDKN2A