Melanocortin-5 R/MC5R Antibody

Western Blot: Melanocortin-5 R/MC5R Antibody [NBP1-36971] - Analysis of MC5 Receptor in Mouse Muscle lysate (35ug protein in RIPA buffer) using NBP1-36971 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Melanocortin-5 R/MC5R Antibody [NBP1-36971] - (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Melanocortin-5 R/MC5R Antibody Summary

• Immunogen: Peptide with sequence PNVKNKSSPCED corresponding to N-Terminus (near) according to NP_005904.1.
• Epitope: N Terminus (near)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MC5R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3-1 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 36.6 kDa band according to NP_005904.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Melanocortin-5 R/MC5R Antibody

• MC2
• MC-2
• MC5
• MC5R
• MC5-R
• melanocortin 5 receptor
• melanocortin receptor 5
• Melanocortin-5 R
• melanocortin-5 receptor
• Melanocortin5R
• Melanocortin-5R

Background

The melanocortin family comprises the alpha-, beta- and gamma-melanocyte stimulating hormones (MSH) and adrenocorticotrophin. The receptors for these hormones are seven-transmembrane, G protein-coupled proteins that activate adenylyl cyclase. Five melanocortin receptors have been cloned and shown to exhibit different affinities and patterns of expression. MC1-R (MSH-R) is expressed in melanocytes and corticoadrenal tissue. MC2-R is the ACTH receptor and is expressed primarily in the adrenal cortex. MC3-R has been found in specific regions of the brain and is also expressed in placenta and gut. MC4-R is expressed primarily in brain, while MC5-R is expressed at low levels in most tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melanocortin-5 R/MC5R Antibody (NBP1-36971)(1)

Publication using NBP1-36971

• Grieco P, Cai M, Han G et al. Further structure-activity studies of lactam derivatives of MT-II and SHU-9119: their activity and selectivity at human melanocortin receptors 3, 4, and 5. Peptides 2007-06-01 [PMID: 17482720]

Bioinformatics

• Gene Symbol: MC5R
• Entrez:
  • 4161(Human)
  • 17203(Mouse)
• Uniprot:
  • NP_005904.1(Human)