Melanocortin-5 R/MC5R Antibody

Images

 
Western Blot: Melanocortin-5 R/MC5R Antibody [NBP1-36971] - Analysis of MC5 Receptor in Mouse Muscle lysate (35ug protein in RIPA buffer) using NBP1-36971 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: Melanocortin-5 R/MC5R Antibody [NBP1-36971] - (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Melanocortin-5 R/MC5R Antibody Summary

Immunogen
Peptide with sequence PNVKNKSSPCED corresponding to N-Terminus (near) according to NP_005904.1.
Epitope
N Terminus (near)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MC5R
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3-1.0 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 37 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 36.6 kDa band according to NP_005904.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-36971.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Melanocortin-5 R/MC5R Antibody

  • MC2
  • MC-2
  • MC5
  • MC5R
  • MC5-R
  • melanocortin 5 receptor
  • melanocortin receptor 5
  • Melanocortin-5 R
  • melanocortin-5 receptor
  • Melanocortin5R
  • Melanocortin-5R

Background

The melanocortin family comprises the alpha-, beta- and gamma-melanocyte stimulating hormones (MSH) and adrenocorticotrophin. The receptors for these hormones are seven-transmembrane, G protein-coupled proteins that activate adenylyl cyclase. Five melanocortin receptors have been cloned and shown to exhibit different affinities and patterns of expression. MC1-R (MSH-R) is expressed in melanocytes and corticoadrenal tissue. MC2-R is the ACTH receptor and is expressed primarily in the adrenal cortex. MC3-R has been found in specific regions of the brain and is also expressed in placenta and gut. MC4-R is expressed primarily in brain, while MC5-R is expressed at low levels in most tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melanocortin-5 R/MC5R Antibody (NBP1-36971)(1)

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Bioinformatics

Gene Symbol MC5R
Entrez
Uniprot