MEK1 Antibody (SZ22-01)

Western Blot: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of MEK1 on different lysates using anti-MEK1 antibody at 1:1000 dilution. Positive control in Lane 1: A431. Lane 2: HepG2. Lane 3: HeLa.

Immunocytochemistry/Immunofluorescence: MEK1 Antibody (SZ22-01) [NBP2-67358] - Staining MEK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of paraffin-embedded mouse uterus tissue using anti-MEK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of HeLa cells with MEK1 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: MEK1 Antibody (SZ22-01) [NBP2-67358] - Whole cell lysates (30 ug per lane) from three different hematological cancer cell lines. Antibody at 1:1000. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: MEK1 Antibody (SZ22-01) [NBP2-67358] - Staining MEK1 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunocytochemistry/Immunofluorescence: MEK1 Antibody (SZ22-01) [NBP2-67358] - Staining MEK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of paraffin-embedded human kidney tissue using anti-MEK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of paraffin-embedded human tonsil tissue using anti-MEK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MEK1 Antibody (SZ22-01) [NBP2-67358] - Analysis of paraffin-embedded mouse pancreas tissue using anti-MEK1 antibody. Counter stained with hematoxylin.

Product Discontinued

MEK1 Antibody (SZ22-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal human MEK1. (SwissProt: Q02750 Human; SwissProt: P31938 Mouse; SwissProt: Q01986 Rat)
• Localization: Cytoplasm, Nucleus, Membrane, Cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP2K1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MEK1 Antibody (SZ22-01)

• dual specificity mitogen-activated protein kinase kinase 1
• ERK activator kinase 1
• MAP kinase kinase 1
• MAP2K1
• MAPK/ERK kinase 1
• MAPKK1
• MEK1
• MEK1MAPKK 1
• mitogen-activated protein kinase kinase 1
• MKK1
• MKK1MEK 1
• PRKMK1EC 2.7.12.2
• protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MEK1 Antibody (NBP2-67358) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: PRABIR CHAKRABORTY WB Human 11/16/2019

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates from hematological cancer cell lines
• Species: Human
• Lot: HL0619

Comments

• Comments: 30 ug protein per sample.
  Primary Ab dilution 1:1000

Bioinformatics

• Gene Symbol: MAP2K1