MED15 Antibody

Western Blot: MED15 Antibody [NBP1-28800] - (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MED15 Antibody Summary

• Immunogen: Peptide with sequence C-PPQSQTQPLVSQAQ corresponding to internal region according to NP_001003891.1, NP_056973.2.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001003891.1; NP_056973.2).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MED15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human lung lysates (calculated MW of 86.8 kDa band according to NP_001003891.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MED15 Antibody

• ARC105Arc105
• CAG7A
• CTG repeat protein 7a
• CTG7A
• FLJ42282
• FLJ42935
• mediator complex subunit 15PC2 glutamine/Q-rich-associated protein
• mediator of RNA polymerase II transcription subunit 15
• PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associatedprotein
• PC2-glutamine-rich-associated protein
• PCQAPDKFZp686A2214
• Positive cofactor 2 glutamine/Q-rich-associated protein
• positive cofactor 2, glutamine/Q-rich-associated protein
• TIG1DKFZp762B1216
• TIG-1TPA-inducible gene 1 protein
• TNRC7
• TPA inducible gene-1
• TPA inducible protein
• trinucleotide repeat containing 7,105-kD
• Trinucleotide repeat-containing gene 7 protein

Background

The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED15 Antibody (NBP1-28800)(1)

Publication using NBP1-28800

• Thakur JK, Arthanari H, Yang F et al. A nuclear receptor-like pathway regulating multidrug resistance in fungi. Nature 2008-04-01 [PMID: 18385733]

Bioinformatics

• Gene Symbol: MED15
• Entrez:
  • 51586(Human)
  • 94112(Mouse)
• Uniprot:
  • NP_001003891.1(Human)