MDM2/HDM2 [p Ser166] Antibody


Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP1-51396] - Analysis of p-MDM2 (S166) pAb at 1:500 dilution Lane1:THP-1 cell lysate treated with EGF(0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with EGF more
Immunohistochemistry-Paraffin: MDM2/HDM2 [p Ser166] Antibody [NBP1-51396] - Analyzes of p-MDM2 (S166) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: MDM2 [p Ser166] Antibody [NBP1-51396] - MDM2 (S166) pAb in extracts from LOVO cells treated with EGF
Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP1-51396] - Analysis of p-MDM2 (S166) pAb at 1:500 dilutionLane1:HEK293T treated with EGF(0.1ng/ml,15min) whole cell lysate Lane2:HEK293T treated with EGF(0.1ng/ml,30min) more
Immunohistochemistry-Paraffin: MDM2 [p Ser166] Antibody [NBP1-51396] Human breast carcinoma tissue.

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MDM2/HDM2 [p Ser166] Antibody Summary

Synthetic phosphopeptide derived from human MDM2 around the phosphorylation site of Serine 166.
p Ser166
p-MDM2 (S166) pAb detects endogenous levels of MDM2 protein only when phosphorylated at S166
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-MDM2 (S166) pAb in extracts from LOVO cells treated with EGFImmunohistochemistry (IHC) analyzes of p-MDM2 (S166) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for MDM2/HDM2 [p Ser166] Antibody

  • HDM2
  • HDMX
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • MDM2


p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wt p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol MDM2