MDM2/HDM2 [p Ser166] Antibody

Images

 
Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP1-51396] - Analysis of p-MDM2 (S166) pAb at 1:500 dilution Lane1:THP-1 cell lysate treated with EGF(0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with EGF ...read more
Immunohistochemistry-Paraffin: MDM2/HDM2 [p Ser166] Antibody [NBP1-51396] - Analyzes of p-MDM2 (S166) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: MDM2 [p Ser166] Antibody [NBP1-51396] - MDM2 (S166) pAb in extracts from LOVO cells treated with EGF
Western Blot: MDM2/HDM2 [p Ser166] Antibody [NBP1-51396] - Analysis of p-MDM2 (S166) pAb at 1:500 dilutionLane1:HEK293T treated with EGF(0.1ng/ml,15min) whole cell lysate Lane2:HEK293T treated with EGF(0.1ng/ml,30min) ...read more
Immunohistochemistry-Paraffin: MDM2 [p Ser166] Antibody [NBP1-51396] Human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related MDM2/HDM2 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51396
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

MDM2/HDM2 [p Ser166] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human MDM2 around the phosphorylation site of Serine 166.
Modification
p Ser166
Specificity
p-MDM2 (S166) pAb detects endogenous levels of MDM2 protein only when phosphorylated at S166
Clonality
Polyclonal
Host
Rabbit
Gene
MDM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-MDM2 (S166) pAb in extracts from LOVO cells treated with EGFImmunohistochemistry (IHC) analyzes of p-MDM2 (S166) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDM2/HDM2 [p Ser166] Antibody

  • ACTFS
  • HDM2
  • HDMX
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • MDM2

Background

p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wt p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP1-87425
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF6897
Species: Hu, Mu
Applications: WB, IHC
NBP1-31308
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
NB100-556
Species: Hu
Applications: WB, IP
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-56598
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
H00056996-R01
Species: Hu
NB600-210
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for MDM2/HDM2 Antibody (NBP1-51396) (0)

There are no publications for MDM2/HDM2 Antibody (NBP1-51396).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MDM2/HDM2 Antibody (NBP1-51396) (0)

There are no reviews for MDM2/HDM2 Antibody (NBP1-51396). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MDM2/HDM2 Antibody (NBP1-51396) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MDM2/HDM2 Products

Bioinformatics Tool for MDM2/HDM2 Antibody (NBP1-51396)

Discover related pathways, diseases and genes to MDM2/HDM2 Antibody (NBP1-51396). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MDM2/HDM2 Antibody (NBP1-51396)

Discover more about diseases related to MDM2/HDM2 Antibody (NBP1-51396).
 

Pathways for MDM2/HDM2 Antibody (NBP1-51396)

View related products by pathway.

PTMs for MDM2/HDM2 Antibody (NBP1-51396)

Learn more about PTMs related to MDM2/HDM2 Antibody (NBP1-51396).
 

Research Areas for MDM2/HDM2 Antibody (NBP1-51396)

Find related products by research area.

Blogs on MDM2/HDM2

There are no specific blogs for MDM2/HDM2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MDM2/HDM2 [p Ser166] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MDM2
Entrez