MDL-1/CLEC5A Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

MDL-1/CLEC5A Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MDL‑1/CLEC5A
  Tyr26-Lys190
  Accession # AAI04365
• Specificity: Detects mouse MDL‑1/CLEC5A in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human MDL-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLEC5A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MDL-1/CLEC5A Antibody [Biotin]

• CLEC5A
• CLECSF5
• CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5
• C-type lectin domain family 5, member A
• C-type lectin superfamily member 5
• MDL1
• MDL-1
• MDL-1MGC138304
• Myeloid DAP12-associating lectin 1
• myeloid DAP12-associating lectin-1

Background

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDL-1/CLEC5A Antibody (BAF1639)(1)

Publication using BAF1639

• Koth LL, Cambier CJ, Ellwanger A DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2. J. Immunol., 2010-04-26;184(11):6522-8. 2010-04-26 [PMID: 20421649] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CLEC5A
• Uniprot:
  • AAI04365()