MDL-1/CLEC5A Antibody (283834) [Allophycocyanin]

Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and (B) Mouse Anti-Human MDL-1/CLEC5A APC-conjugated Monoclonal Antibody (Catalog # FAB2384A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 283834
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

MDL-1/CLEC5A Antibody (283834) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
  Tyr26-Lys188
  Accession # Q9NY25
• Specificity: Detects human MDL‑1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL‑1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC5A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MDL-1/CLEC5A Antibody (283834) [Allophycocyanin]

• CLEC5A
• CLECSF5
• CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5
• C-type lectin domain family 5, member A
• C-type lectin superfamily member 5
• MDL1
• MDL-1
• MDL-1MGC138304
• Myeloid DAP12-associating lectin 1
• myeloid DAP12-associating lectin-1

Background

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily. MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDL-1/CLEC5A Antibody (FAB2384A)(2)

Publications using FAB2384A

• KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman Identification of HIV transmitting CD11c+ human epidermal dendritic cells Nat Commun, 2019-06-21;10(1):2759. 2019-06-21 [PMID: 31227717] (Flow Cytometry, Human)
• RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes J. Leukoc. Biol, 2017-03-07;0(0):. 2017-03-07 [PMID: 28270408] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CLEC5A
• Uniprot:
  • Q9NY25()