MAPKAP Kinase 3 Antibody (2B5)

Western Blot: MAPKAP Kinase 3 Antibody (2B5) [H00007867-M02] - MAPKAPK3 monoclonal antibody (M02), clone 2B5 Analysis of MAPKAPK3 expression in HeLa.

Immunohistochemistry-Paraffin: MAPKAP Kinase 3 Antibody (2B5) [H00007867-M02] - Analysis of monoclonal antibody to MAPKAPK3 on FFPE human placenta. Antibody concentration 3 ug/mL.

Western Blot: MAPKAP Kinase 3 Antibody (2B5) [H00007867-M02] - MAPKAPK3 monoclonal antibody (M02), clone 2B5. Analysis of MAPKAPK3 expression in NIH/3T3.

Proximity Ligation Assay: MAPKAP Kinase 3 Antibody (2B5) [H00007867-M02] - Analysis of protein-protein interactions between MAPK13 and MAPKAPK3. HeLa cells were stained with anti-MAPK13 rabbit purified polyclonal 1:1200 and anti-MAPKAPK3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: MAPKAP Kinase 3 Antibody (2B5) [H00007867-M02] - Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.03ng/mL as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Func, IHC
• Clone: 2B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MAPKAP Kinase 3 Antibody (2B5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAPKAPK3 (AAH01662, 272 a.a. ~ 382 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ
• Specificity: MAPKAPK3 - mitogen-activated protein kinase-activated protein kinase 3
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPKAPK3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAPKAP Kinase 3 Antibody (2B5)

• 3PKEC 2.7.11.1
• Chromosome 3p kinase
• EC 2.7.11
• MAP kinase-activated protein kinase 3
• MAPK-activated protein kinase 3
• MAPKAP kinase 3
• MAPKAP33pK
• MAPKAPK-3
• mitogen-activated protein kinase-activated protein kinase 3

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAPK3
• Entrez:
  • 7867(Human)
• OMIM:
  • 602130(Human)
• Uniprot:
  • AAH01662(Human)