MafK Antibody (381923) [DyLight 405]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 381923
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 405

MafK Antibody (381923) [DyLight 405] Summary

• Immunogen: E. coli-derived recombinant human MafK
  Thr2-Ser156
  Accession # O60675
• Specificity: Detects human and mouse MafK in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafB or rhMafF is observed. In Western blots, prolonged film exposures may slightly detect rhMafG.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAFK
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MafK Antibody (381923) [DyLight 405]

• FLJ32205
• MafK
• MGC71717
• NFE2U
• transcription factor MafK
• ubiquitous (p18)
• v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K
• v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein K
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)

Background

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 177000). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAFK