MafK Antibody (2F7) - BSA Free

Western Blot: MafK Antibody (2F7) [NBP2-22570] - Western Blot: MafK Antibody (2F7) [NBP2-22570] - The recombinant proteins (20ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with anti human MAFK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human MAFKLane 2. : Recombinant Human MAFGLane 3. : Recombinant Human MAFF

Western Blot: MAFK Antibody (2F7) [NBP2-22570] - The cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAFK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MafK Antibody (2F7) - BSA Free Summary

• Description: Novus Biologicals Mouse MafK Antibody (2F7) - BSA Free (NBP2-22570) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human MAFK (1-156aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAFK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MafK Antibody (2F7) - BSA Free

• FLJ32205
• MafK
• MGC71717
• NFE2U
• transcription factor MafK
• ubiquitous (p18)
• v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K
• v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein K
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)

Background

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteins is primarily expressed during development in mesenchymal and hematopoietic cells and neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAFK
• Uniprot:
  • NP_002351(Human)