Macro H2A.2 Antibody (CL5263) - Azide and BSA Free

Staining of human kidney shows strong nuclear positivity in cells in renal glomeruli and a subset of renal tubules.

Staining of human fallopian tube shows strong nuclear immunoreactivity in glandular cells and moderate positivity in stromal cells.

Staining of human cerebral cortex shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.

Staining of PC-3 cells using the Anti-Macro H2A.2 monoclonal antibody) .

Staining of human testis shows strong nuclear immunoreactivity in a subset of cells in seminiferous tubules.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL5263
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Macro H2A.2 Antibody (CL5263) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of Q9P0M6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MACROH2A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Macro H2A.2 Antibody (CL5263) - Azide and BSA Free

• core histone macro-H2A.2
• core histone macroH2A2.2
• H2A histone family, member Y2
• Histone macroH2A2
• macroH2A2
• mH2A2

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MACROH2A2