Macro H2A.2 Antibody (CL5263) - BSA Free

Immunocytochemistry/Immunofluorescence: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Staining of PC-3 cells using the Anti-H2AFY2 monoclonal antibody, showing specific staining in the nucleoplasm and nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in renal glomeruli and a subset of renal tubules.

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Immunohistochemical staining of human testis shows strong nuclear immunoreactivity in a subset of cells in seminiferous tubules.

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Immunohistochemical staining of human fallopian tube shows strong nuclear immunoreactivity in glandular cells and moderate positivity in stromal cells.

Immunohistochemistry: Macro H2A.2 Antibody (CL5263) [NBP2-61156] - Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL5263
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Macro H2A.2 Antibody (CL5263) - BSA Free Summary

• Immunogen: This antibody was developed using a synthetic peptide derived from Q9P0M6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MACROH2A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Macro H2A.2 Antibody (CL5263) - BSA Free

• core histone macro-H2A.2
• core histone macroH2A2.2
• H2A histone family, member Y2
• Histone macroH2A2
• macroH2A2
• mH2A2

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MACROH2A2