Lysyl tRNA synthetase Antibody (JE40-55)

Western Blot: Lysyl tRNA synthetase Antibody (JE40-55) [NBP2-75560] - Analysis of Lysyl tRNA synthetase on SiHa (1) and MCF-7 (2) cell lysate using anti-Lysyl tRNA synthetase antibody at 1/2,000 dilution.

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody (JE40-55) [NBP2-75560] - Analysis of paraffin-embedded rat testis tissue using anti-Lysyl tRNA synthetase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody (JE40-55) [NBP2-75560] - Analysis of paraffin-embedded human colon tissue using anti-Lysyl tRNA synthetase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody (JE40-55) [NBP2-75560] - Analysis of paraffin-embedded human prostate cancer tissue using anti-Lysyl tRNA synthetase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody (JE40-55) [NBP2-75560] - Analysis of paraffin-embedded mouse brain tissue using anti-Lysyl tRNA synthetase antibody. Counter stained with hematoxylin.

Product Discontinued

Lysyl tRNA synthetase Antibody (JE40-55) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Lysyl tRNA synthetase aa 460-597 / 597. (SwissProt: Q15046 Human; SwissProt: Q99MN1 Mouse; SwissProt: Q5XIM7 Rat)
• Localization: Cytoplasm.Cell membrane. Mitochondrion. Nucleus. Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KARS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Lysyl tRNA synthetase Antibody (JE40-55)

• CMTRIB
• EC 6.1.1
• EC 6.1.1.6
• KARS2
• KIAA0070
• KRS
• lysine tRNA ligase
• Lysine--tRNA ligase
• lysRS
• lysyl-tRNA synthetase

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KARS1