Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human KDM5B
  Leu819-Arg918
  Accession # Q9UGL1
• Specificity: Detects human KDM5B in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [Alexa Fluor® 350]

• CT31
• CT31FLJ10538
• EC 1.14.11
• EC 1.14.11.-
• homolog 1A
• Jarid1B
• JARID1BFLJ16281
• Jumonji, AT rich interactive domain 1B (RBP2-like)
• jumonji, AT rich interactive domain 1B
• KDM5B
• Lysine (K)specific Demethylase 5B
• Lysine (K)-specific Demethylase 5B
• PLU-1
• PLU-1FLJ12459
• PLU1FLJ23670
• PUT1
• RBBP2H1
• RBBP2H1AFLJ12491
• RBP2-H1
• Retinoblastoma-binding protein 2 homolog 1

Background

KDM5B (also JARID1B, RBP2-H1 and PLU-1) is a 170-200 kDa member of the JARID1 histone demethylase family of proteins. It is a transcriptional repressor that is highly expressed in Sertoli cells and mammary epithelium during pregnancy, and interacts with BF-1 and PAX9. KDM5B is reported to demethylate tri- and di-methylated Lys4 on histone H3. It has no activity on Lys9 or 27. Human KDM5B is 1544 amino acids (aa) in length. It contains a jumonji N domain (aa 32-73), an ARID DNA binding region (aa 97-187), a PHD domain (aa 309-359), a jumonji C domain (aa 486-602), one C5HC2 motif (aa 692-745), and two consecutive PHD domains (aa 1176-1538). There are three utilized phosphorylation sites at Ser1383, 1384 and 1405.There are three potential splice variants. Two show a premature truncation after Pro1433 coupled to an alternative start site at either Met396 or Met159. A third contains a 36 aa insertion after Glu237. Over aa 819-918, human KDM5B shares 96% aa identity with mouse KDM5B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.