Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Product Discontinued

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein (NM_006618).
• Specificity: This product is specific for Human JARID1B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA and Western Blot. Use in FLOW was reported in the scientific literature (PMID: 23764003). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30472020).

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

• CT31
• CT31FLJ10538
• EC 1.14.11
• EC 1.14.11.-
• homolog 1A
• Jarid1B
• JARID1BFLJ16281
• Jumonji, AT rich interactive domain 1B (RBP2-like)
• jumonji, AT rich interactive domain 1B
• KDM5B
• Lysine (K)specific Demethylase 5B
• Lysine (K)-specific Demethylase 5B
• PLU-1
• PLU-1FLJ12459
• PLU1FLJ23670
• PUT1
• RBBP2H1
• RBBP2H1AFLJ12491
• RBP2-H1
• Retinoblastoma-binding protein 2 homolog 1

Background

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (22260002)(7)

Publications using 22260002

• Hinohara K, Wu HJ, Vigneau S et al. KDM5 Histone Demethylase Activity Links Cellular Transcriptomic Heterogeneity to Therapeutic Resistance. Cancer Cell. 2018-11-14 [PMID: 30472020] (Chemotaxis, Human)
• Noguchi F, Inui S, Fedele C et al. Calcium-dependent enhancement by extracellular acidity of the cytotoxicity of mitochondrial inhibitors against melanoma. Mol. Cancer Ther. 2017-02-21 [PMID: 28223427] (FLOW, Human)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Yuan P, Ito K, Perez-Lorenzo R et al. Phenformin enhances the therapeutic benefit of BRAFV600E inhibition in melanoma. Proc Natl Acad Sci U S A. 2013-11-05 [PMID: 24145418] (FLOW, Human)
• Roesch A, Vultur A, Bogeski I et al. Overcoming Intrinsic Multidrug Resistance in Melanoma by Blocking the Mitochondrial Respiratory Chain of Slow-Cycling JARID1Bhigh Cells. Cancer Cell 2013-06-10 [PMID: 23764003] (FLOW, Human)
• Strizzi L, Margaryan NV, Gilgur A et al. The significance of a Cripto-1 positive subpopulation of human melanoma cells exhibiting stem cell-like characteristics. Cell Cycle 2013-05-01 [PMID: 23574716] (WB, Human)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: KDM5B
• Entrez:
  • 10765(Human)
• Uniprot:
  • Q9UGL1()