LYNX1 Overexpression Lysate (Native)

Images

 
Western Blot: LYNX1 Overexpression Lysate (Adult Normal) [NBL1-12755] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LYNX1.
Western Blot: LYNX1 Overexpression Lysate (Adult Normal) [NBL1-12755] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for LYNX1

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

LYNX1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207126 and based on accession number NM_023946. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Ly6/neurotoxin 1 (LYNX1), transcript variant 1, mRNA.
Gene
LYNX1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for LYNX1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LYNX1 Overexpression Lysate (Native)

  • Ly-6 neurotoxin-like protein 1
  • Ly6/neurotoxin 1
  • ly-6/neurotoxin-like protein 1
  • MGC40364
  • secreted Ly6/uPAR related protein 2
  • secreted Ly-6/uPAR-related protein 2
  • SLURP2
  • SLURP-2

Background

This gene encodes a member of the Ly-6/neurotoxin gene family, a group of lymphocyte antigens that attach to the cell surface by a glycosylphosphatidylinositol anchor and have a unique structure showing conserved 8-10 cysteine residues with a characteristic spacing pattern. Functional analysis indicates that this protein is not a ligand or neurotransmitter but has the capacity to enhance nicotinic acetylcholine receptor function in the presence of acetylcholine. This gene may also play a role in the pathogenesis of psoriasis vulgaris. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol LYNX1