LSM4 Antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-LSM4 )

ELISA: LSM4 Antibody [NB120-19101] - Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/ml) and serial dilutions of antibody. ( anti-LSM4 )

Product Discontinued

LSM4 Antibody Summary

• Immunogen: Recombinant fragment, corresponding to amino acids 1-139 of Human LSM4.
• Localization: Nuclear
• Specificity: LSM4
• Clonality: Polyclonal
• Host: Chicken
• Gene: LSM4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ICC: 1/200. Western Blot: 1/500. Predicted molecular weight: 15 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: IgG purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for LSM4 Antibody

• Glycine-rich protein
• GRP
• LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• U6 snRNA-associated Sm-like protein LSm4
• YER112W

Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LSM4
• Entrez:
  • 25804(Human)
  • 50783(Mouse)
  • 290647(Rat)