LR8 Antibody

Western Blot: LR8 Antibody [NBP2-13446] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunocytochemistry/Immunofluorescence: LR8 Antibody [NBP2-13446] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.

Immunohistochemistry-Paraffin: LR8 Antibody [NBP2-13446] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Product Discontinued

LR8 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VGLRNLCGQSSQPLNEEGSEKRLLGENSVPPSPSREQTSTAIVL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM176B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for LR8 Antibody

• LR8
• LR8FLJ26014
• LR8-like protein
• MGC110857
• Protein LR8
• TMEM176B
• transmembrane protein 176B

Background

LR8 may play a role in the process of maturation of dendritic cells. Required for the development of cerebellar granule cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMEM176B