LIR-8/CD85c/LILRB5 Antibody [Unconjugated]

Human peripheral blood monocytes were stained with Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3065, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Western blot shows lysates of Human bone marrow. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for LILRB5/CD85c/LIR-8 at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LIR-8/CD85c/LILRB5 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8
  Arg18-His456
  Accession # O75023
• Specificity: Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LILRA4/ILT7 is observed and less than 15% cross-reactivity with rhILT3 and rhILT5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LILRB5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1-0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIR-8/CD85c/LILRB5 Antibody [Unconjugated]

• CD85 antigen-like family member C
• CD85c antigen
• CD85c
• Leukocyte immunoglobulin-like receptor 8
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRB5
• LIR8
• LIR-8
• LIR8CD85C
• LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5
• member 5

Background

Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIR-8/CD85c/LILRB5 Antibody (AF3065)(4)

Publications using AF3065

• M Comi, D Avancini, F Santoni de, M Villa, MJ Uyeda, M Floris, D Tomasoni, A Bulfone, MG Roncarolo, S Gregori Coexpression of CD163 and CD141 identifies human circulating IL-10-producing dendritic cells (DC-10) Cell. Mol. Immunol., 2019-03-06;0(0):. 2019-03-06 [PMID: 30842629] (Flow Cytometry, Human)
• The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation Nat Commun, 2016-05-05;7(0):11473. 2016-05-05 [PMID: 27145901] (Flow Cytometry, Human)
• Zhang Z, Hatano H, Shaw J, Olde Nordkamp M, Jiang G, Li D, Kollnberger S The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains. PLoS ONE, 2015-06-22;10(6):e0129063. 2015-06-22 [PMID: 26098415] (Flow Cytometry, Human)
• Zheng J, Umikawa M, Cui C, Li J, Chen X, Zhang C, Hyunh H, Kang X, Silvany R, Wan X, Ye J, Canto AP, Chen SH, Wang HY, Ward ES, Zhang CC Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development. Nature, 2012-05-30;485(7400):656-60. 2012-05-30 [PMID: 22660330] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: LILRB5
• Uniprot:
  • O75023()