Lgr4/GPR48 Antibody

Images

 
Immunohistochemistry: Lgr4/GPR48 Antibody [NBP1-89737] - Staining of human breast shows strong positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Lgr4/GPR48 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:KSHSCPALAVASCQRPEGYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (95%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LGR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Lgr4/GPR48 Protein (NBP1-89737PEP)
Publications
Read Publication using NBP1-89737.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lgr4/GPR48 Antibody

  • GPR48
  • GPR48G protein-coupled receptor 48
  • G-protein coupled receptor 48
  • leucine-rich repeat containing G protein-coupled receptor 4
  • Lgr4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lgr4/GPR48 Antibody (NBP1-89737)(1)

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Product General Protocols

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FAQs for Lgr4/GPR48 Antibody (NBP1-89737). (Showing 1 - 1 of 1 FAQ).

  1. May we ask why there are so many different MW expressions detected on the WB image of NBP1-89737? Can we expect to obtain GPR48 signals at approximately 104.5 KDa?
    • GPR48 protein and multi-pass transmembrane proteins in general are not easy to work with in WB assay and requires extensive optimization. This protein has several glycosylation sites and form di-sulphide multimers and can aggregate when heated during sample preparation, so that customer may see variation in observed vs expected molecular weight. We highly recommend employing Urea-gels for WB assay of this protein to deal with aggregate problems. GPR48, catalog number NBP1-89737, has been approved for Western Blot based on the band of approximately 105kD observed in RT-4 as well as U-251 MG cell lysates along with additional bands at lower position that we have not characterized yet. In our QC setting, unless required crucially, the Western blot analysis for each antibody is performed using an identical set up i.e. the same gel type and conditions are used for all antibodies and when membranes are incubated with antibodies long enough or develop the blot for a long time, there is a probability that you will see extra bands at different levels. We always show the original results achieved from lysates tested and do not remove lanes with extra bands that can not be explained with predicted target weights.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LGR4
Entrez
Uniprot