Lgr4/GPR48 Antibody (852229) [Phycoerythrin]

• Applications: Flow
• Clone: 852229
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Lgr4/GPR48 Antibody (852229) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Lgr4/GPR48
  Met1-Asp951
  Accession # Q9BXB1
• Specificity: Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGR4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Lgr4 (Leucine-rich repeat GPR 4), also known as GPR48 (G-Protein-coupled Receptor 48), is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and hCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein-independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr4 binds and forms complexes with R-Spondins, Frizzled Wnt receptors, and LRP Wnt co-receptors (5). It acts, at least in part, by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta -catenin (3, 4). Human Lgr4 cDNA encodes 951 amino acids (aa), including a long N-terminal Extracellular Domain (ECD, aa 25-544) with 16-17 LRR domains that mediate ligand interaction (1). The LRR-containing ECD of human Lgr4 shares 93% aa sequence identity with mouse, rat and bovine Lgr4, and 50-60% aa identity with human Lgr5 and Lgr6. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7-9). Lgr4 deletion in the mouse affects development in areas of normal expression, for example, it will inhibit fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over-expressed in carcinomas and may promote invasiveness and metastasis by down-regulating p27^Kip1 expression (10).

1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.
2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.
5. de Lau, W. et al. (2011) Nature 476:293.
6. Ruffner, H. et al. (2012) PLoS ONE 7:e40975.
7. Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.
8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.
9. Song, H. et al. (2008) J. Biol. Chem. 283:36687.
10. Gao, Y. et al. (2006) Cancer Res. 66:11623.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR4