Lamin B1 Antibody

Product Discontinued

Lamin B1 Antibody Summary

• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B,different from the related rat and mouse sequence by three amino acids.
• Marker: Nuclear Envelope Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LMNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot 0.1-0.5ug/ml
• Theoretical MW: 66.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, and 0.2mg Na2HPO4
• Preservative: 0.05mg Thimerosal, 0.05mg Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.2 ml distilled water to a final concentration of 0.5 mg/ml.

Alternate Names for Lamin B1 Antibody

• ADLD
• Lamin B1
• lamin-B1
• LMN
• LMN2
• LMNB
• LMNB1
• MGC111419

Background

The lamins form a meshwork of filaments on the inner, or nucleoplasmic, surface of the nuclear envelope. Currently, mammalian species appear to contain at least four major lamins, called A, B1, B2, and C, of which two (A and C) arise by alternative splicing of the same gene and two minor lamins, D and E, whose molecular weight varies from species to species. Nuclei of embryonic and lymphiod cells contain only lamin B1. Mutations affecting A-type lamins have been associated with a variety of human diseases, including muscular dystrophy, cardiomyopathy, lipodystrophy, and progeria, but mutations in B-type lamins have never been identified in humans. Homozygous lamin B1 mutant mice survive embryonic development but died at birth with defects in lung and bone. Cells obtained from mutant lamin B1 mice are defective in differentiation, polyploidy and senesce prematurely.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Lamin B1 Antibody (NBP1-42594)(2)

Publications using NBP1-42594

• Yoon JH, Shin JW, Pham TH Methyl lucidone induces apoptosis and G2/M phase arrest via the PI3K/Akt/NF-kappa B pathway in ovarian cancer cells Pharm Biol 2020-12-01 [PMID: 31875458] (WB, Human)

FAQs for Lamin B1 Antibody (NBP1-42594). (Showing 1 - 2 of 2 FAQs).

1. I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?
   • We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.
2. Is this antibody lyophilized? I have received it as solid material. Am I meant to add water to it?
   • The lab has informed me that NBP1-42594 is lyophilized. Before use, please add 0.2 ml of distilled water, to yield a concentration of 500 ug/ml. After reconstitution, the lab suggests storing at 4 C for one month. It can also be aliquotted and stored at -20 C for longer time.

Bioinformatics

• Gene Symbol: LMNB1
• Entrez:
  • 4001(Human)
• Uniprot:
  • P20700()