Kv3.4 Antibody (S72-16)

Western Blot: Kv3.4 Antibody (S72-16) [NBP1-47609] - analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry-Paraffin: Kv3.4 Antibody (S72-16) [NBP1-47609] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrinlike staining.

Immunohistochemistry-Paraffin: Kv3.4 Antibody (S72-16) [NBP1-47609] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli .

Product Discontinued

Kv3.4 Antibody (S72-16) Summary

• Immunogen: Synthetic peptide amino acids 175-192 of rat Kv3.4
• Epitope: (GDEAGDDERELALQRLGP)
• Localization: Membrane
• Specificity: Detects approx 70kDa (100kDa in brain due to glycosylation).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNC4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of Kv3.4 Antibody was sufficient for detection of Kv3.4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Kv3.4 Antibody (S72-16)

• HKSHIIIC
• K+ channel subunit
• KSHIIIC
• KV3.4
• MGC126818
• potassium voltage-gated channel subfamily C member 4
• potassium voltage-gated channel, Shaw-related subfamily, member 4
• Voltage-gated potassium channel subunit Kv3.4

Background

KCNC4 (Potassium voltage gated channel subfamily C member 4) belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNC4
• Entrez:
  • 684516(Rat)
• Uniprot:
  • Q63734(Rat)