Kv3.4 Antibody (S72-16)

Images

 
Western Blot: Kv3.4 Antibody (S72-16) [NBP1-47609] - analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16. ...read more
Immunohistochemistry-Paraffin: Kv3.4 Antibody (S72-16) [NBP1-47609] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal ...read more
Immunohistochemistry-Paraffin: Kv3.4 Antibody (S72-16) [NBP1-47609] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
S72-16
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Kv3.4 Antibody (S72-16) Summary

Immunogen
Synthetic peptide amino acids 175-192 of rat Kv3.4
Epitope
(GDEAGDDERELALQRLGP)
Localization
Membrane
Specificity
Detects approx 70kDa (100kDa in brain due to glycosylation).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KCNC4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of Kv3.4 Antibody was sufficient for detection of Kv3.4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Kv3.4 Antibody (S72-16)

  • HKSHIIIC
  • K+ channel subunit
  • KSHIIIC
  • KV3.4
  • MGC126818
  • potassium voltage-gated channel subfamily C member 4
  • potassium voltage-gated channel, Shaw-related subfamily, member 4
  • Voltage-gated potassium channel subunit Kv3.4

Background

KCNC4 (Potassium voltage gated channel subfamily C member 4) belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KCNC4
Entrez
Uniprot