KOR Antibody (387310) [Unconjugated]

KOR was detected in immersion fixed paraffin-embedded sections of human brainstem (medulla) using 15 µg/mL Mouse Anti-Human KOR Monoclonal Antibody (Catalog # MAB38951) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 387310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

KOR Antibody (387310) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human KOR
  Met1-Val380
  Accession # P41145
• Specificity: Detects human KOR. Stains human KOR transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OPRK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KOR Antibody (387310) [Unconjugated]

• kappa opioid receptor
• kappa-type opioid receptor
• KOR
• K-OR-1
• KOR-1
• Opiate receptor, kappa-1
• opioid receptor, kappa 1
• OPRK
• OPRK1
• Oprk2
• R21

Background

Kappa Opioid Receptor (KOR) is a 45 kDa 7TM opioid receptor that is primarily expressed in the central nervous system and peripheral visceral pain sensory nerves. Following ligation by dynorphin peptides, KOR signaling induces analgesia, dysphoria, diuresis, and increased feeding desire. KOR also exerts neuroprotective and anti-inflammatory effects. Human KOR shares 94% amino acid sequence identity with mouse and rat KOR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KOR Antibody (MAB38951)(1)

Publication using MAB38951

• D Chen, Y Chen, Y Yan, J Pan, W Xing, Q Li, W Zeng Down-regulation of the tumour suppressor ?-opioid receptor predicts poor prognosis in hepatocellular carcinoma patients BMC Cancer, 2017-08-18;17(1):553. 2017-08-18 [PMID: 28821282] (IHC-P, Human)

Bioinformatics

• Gene Symbol: OPRK1
• Uniprot:
  • P41145()