KDEL Antibody

Product Discontinued

KDEL Antibody Summary

• Immunogen: Synthetic peptide: C T(643)GEEDTSEKDEL(654). Immunizing peptide corresponds to amino acid residues 643-654 from rat GRP 78.
• Marker: Endoplasmic Reticulum Marker
• Specificity: KDEL
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POGLUT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Western blot: use at concentration of 2 ug/ml.Immunofluorescence: assay dependent.Immunoprecipitation: assay dependent.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP 78) and an ~94 kDa protein representing GRP 94 in mouse myeloma cells. Immunofluorescence staining of GRP 78 in mouse myeloma cells results in diffuse cytoplasmic staining consistent with specific ER localization. This antibody immunoprecipitates PDI (GRP 78), an ~72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ~94 kDa protein representing GRP 94 from numerous rodent tissues.

Reactivity Notes

Cross-reacts with Mouse and Rat. Does not cross-react with Human or African Green Monkey (COS cells). Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for KDEL Antibody

• Endoplasmic reticulum resident protein 58
• EP58KDEL1
• ER protein 58
• ERp58
• KDEL (Lys-Asp-Glu-Leu) containing 1
• KDEL motif-containing 1
• KDEL motif-containing protein 1
• MGC5302

Background

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POGLUT2
• Entrez:
  • 72050(Mouse)
  • 316370(Rat)