KDEL Antibody (10C3) - BSA Free

Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).

Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - KDEL in human primary fibroblasts. Antibody at 1:600 in PBST, 0.1% triton + 10% DS. O/N incubation at 4C. Donkey anti-mouse Alexa Fluor 488 at 1:500 in PBST, 0.1% triton + 10% DS 1h RT. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469] - Analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10 ug/mL.

Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - Analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12.

• Reactivity: Hu, Mu, Rt, Av, Ch, In, Ma, Pl, Pm, Ye
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, MS
• Clone: 10C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

KDEL Antibody (10C3) - BSA Free Summary

• Description: Novus Biologicals Mouse KDEL Antibody (10C3) - BSA Free (NBP1-97469) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. Anti-KDEL Antibody: Cited in 32 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
• Marker: Endoplasmic Reticulum Marker
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POGLUT2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10 - 1:500
  • ELISA
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:34576258).
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Chicken reactivity reported in (PMID: 12163544). Primate reactivity reported in (PMID:12368856). Mouse reactivity reported in (PMID: 18547789). Rat reactivity reported in (PMID: 12969271).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for KDEL Antibody (10C3) - BSA Free

• Endoplasmic reticulum resident protein 58
• EP58KDEL1
• ER protein 58
• ERp58
• KDEL (Lys-Asp-Glu-Leu) containing 1
• KDEL motif-containing 1
• KDEL motif-containing protein 1
• MGC5302

Background

The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KDEL Antibody (NBP1-97469)(33)

Publications using NBP1-97469

• Song Z, Thepsuwan P, Hur WS et al. Regulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD Nature Communications 2024-10-26 [PMID: 39455574]
• Xiaoqiong Wei, You Lu, Liangguang Leo Lin, Chengxin Zhang, Xinxin Chen, Siwen Wang, Shuangcheng Alivia Wu, Zexin Jason Li, Yujun Quan, Shengyi Sun, Ling Qi Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis. Nature communications 2024-01-24 [PMID: 38253565]
• Wu, SA;Shen, C;Wei, X;Zhang, X;Wang, S;Chen, X;Torres, M;Lu, Y;Lin, LL;Wang, HH;Hunter, AH;Fang, D;Sun, S;Ivanova, MI;Lin, Y;Qi, L; The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes Nature communications 2023-05-30 [PMID: 37253728] (Immunocytochemistry/ Immunofluorescence, Mouse)
• Jacquier A, Risson V, Simonet T et al. Severe congenital myasthenic syndromes caused by agrin mutations affecting secretion by motoneurons Acta neuropathologica 2022-08-10 [PMID: 35948834] (ICC/IF)
  
  Details:
  Dilutions: 1:400
• Maddineni P, Kasetti RB, Kodati B et al. Sodium 4-Phenylbutyrate Reduces Ocular Hypertension by Degrading Extracellular Matrix Deposition via Activation of MMP9 International journal of molecular sciences 2021-09-18 [PMID: 34576258] (ICC/IF, Mouse)
• Coudert L, Osseni A, Gangloff Y Et Al. The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways BMC Biology 2021-12-01 [PMID: 34330273] (ICC/IF)
• Wang G, Xu J, Zhao J et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice Nat Commun 2020-01-10 [PMID: 31924786] (IF/IHC, ICC/IF, Mouse)
• Chang MH, Brown MR, Liu Y et al. Cl- and H+ coupling properties and subcellular localizations of wildtype and disease-associated variants of the voltage-gated Cl-/H+ exchanger ClC-5 J. Biol. Chem. 2019-12-18 [PMID: 31852738] (ICC/IF, Human)
• Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep 2019-02-07 [PMID: 30723259] (IHC-P, Mouse)
• Hao Y, Chow AW, Yip WC et al. G protein-coupled estrogen receptor inhibits the P2Y receptor-mediated Ca(2+) signaling pathway in human airway epithelia. Pflugers Arch. 2016-06-06 [PMID: 27271044] (ICC/IF, Human)
• Kilaparty SP, Agarwal R, Singh P et al. Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response. Cell Stress Chaperones. 2016-04-02 [PMID: 27038811] (WB, Mouse)
  
  Details:
  KDEL monoclonal antibody was used for WB analysis of microsomal and cytosolic fractions of MC3T3-E1 cells which were incubated or not with 100 uM etoposide for 24 hours. The antibody detected 75 and 58 kDa bands - the 75 kDa protein was distributed in b
• S Tan et al. Nedd4 Family-interacting Protein 1 (Ndfip1) Is Required for the Exosomal Secretion of Nedd4 Family Proteins. J. Biol. Chem. 283, 32621 . 2008-01-01 [PMID: 18819914] (ICC/IF, Human)
• D Sitterlin et al. The Hepatitis B Virus Precore Protein Is Retrotransported from Endoplasmic Reticulum (ER) to Cytosol through the ER-associated Degradation Pathway. J. Biol. Chem. 283, 32352 . 2008-01-01 [PMID: 18805786] (IP, Human)
• Kurosawa K, Nakano M, Yokoseki I et al. ncBAF, a chromatin remodeler, enhances PXR-mediated transcriptional activation in the human and mouse liver bioRxiv 2023-02-05
• Muroi H, Hori K, Tokutake Y et al. Oleuropein suppresses mitochondrial reactive oxygen species generation possibly via an activation of transient receptor potential V1 and sirtuin-1 in cultured chicken muscle cells Animal science journal = Nihon chikusan Gakkaiho 2022-01-14 [PMID: 35029000] (ICC/IF, Chicken)
• Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked). [PMID: 18755873]
• Y Saga et al. Pofut1 is required for the proper localization of the Notch receptor during mouse development. Mech. Dev. 125, 663. 2008-01-01 [PMID: 18547789] (ICC/IF, Mouse)
• D Ito et al. Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases. Ann. Neurol. 61, 237. 2007-01-01 [PMID: 17387721] (WB, Human)
• B Ulmasov et al. Tissue and subcellular distribution of CLIC1. BMC Cell Biol. 8, 8. 2007-01-01 [PMID: 17326840]
• HM Prehn et al. The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis. J. Neurochem. 87, 248. 2003-01-01 [PMID: 12969271] (WB, Rat)
• RC Austin et al. Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity. J. Biol. Chem. 278, 17438. 2003-01-01 [PMID: 12621026] (WB, Human)
• J Culi & RS Mann. Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila. Cell 112, 343. 2003-01-01 [PMID: 12581524]
• Serwold T, Gonzalez F, Kim J. ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum. Nature 419, 480. 2002-01-01 [PMID: 12368856] (WB, ICC/IF, Primate)
• AS Robinson et al. Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae. Biotechnol. Prog. 18, 942. 2002-01-01 [PMID: 12363344] (WB, Yeast)
• SE Dryer et al. Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion. J. Neurophysiol. 88, 954. 2002-01-01 [PMID: 12163544] (ICC/IF, Chicken)
• R Finnern et al. Efficacy of plant-produced recombinant antibodies against HCG. Hum. Reprod. 17, 2054. 2002-01-01 [PMID: 12151436] (WB, Plant)
• WC Wigley et al. A protein sequence that can encode native structure by disfavoring alternate conformations. Nat. Struct. Biol. 9, 381. 2002-01-01 [PMID: 11938353]
• K Toyooka et al. Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds. J. Cell Biol. 148, 453. 2000-01-01 [PMID: 10662772]
• VI Titorenko & RA Rachubinski. Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis. Mol. Cell Biol. 18, 2789. 1998-01-01 [PMID: 9566898]
• A Choukhi et al. Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins. J. Virol. 72, 3851. 1998-01-01 [PMID: 9557669]
• AA Scheel & HR Pelham. Identification of amino acids in the binding pocket of the human KDEL receptor. J. Biol. Chem. 273, 2467. 1998-01-01 [PMID: 9442098]
• D Vaux et al. Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal. Nature 345, 495. 1990-01-01 [PMID: 2161500]
• HR Pelham. The retention signal for soluble proteins of the endoplasmic reticulum. Trends Biochem. Sci. 15, 483. 1990-01-01 [PMID: 2077689]

Reviews for KDEL Antibody (NBP1-97469) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/31/2020

Summary

• Application: Western Blot
• Sample Tested: Human primary fibroblast
• Species: Human
• Lot: 08071825

Comments

• Comments: Primary antibody 1:1000 incubation O/N 4ºC in Milk 5% in PBST (0.1% Tween)

reviewed by: Verified Customer ICC Human 02/25/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: Human primary fibroblast
• Species: Human
• Lot: 08071825

Comments

• Comments: Great ab for ICC-IF in human fibroblasts. Ab dilution 1:600 (higher dilution needed) in PBST (0.1% triton) + 10% DS. O/N incubation at 4ºC. Secondary Donkey anti-mouse Alexa fluor 488 1:500 in PBST (0.1% triton) + 10% DS 1h RT.

Bioinformatics

• Gene Symbol: POGLUT2