KAT1 Antibody (S04-3G4)

Western Blot: KAT1 Antibody (S04-3G4) [NBP3-19521] - Western blot detection of KAT1/HAT1 in K562, C6, 3T3, Hela cell lysates using KAT1/HAT1 Rabbit mAb (1:1000 diluted). Predicted band size: 50kDa. Observed band size: 45kDa.

Immunohistochemistry-Paraffin: KAT1 Antibody (S04-3G4) [NBP3-19521] - Immunohistochemistry of KAT1/HAT1 in paraffin-embedded Human tonsil using KAT1/HAT1 Rabbit mAb at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S04-3G4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

KAT1 Antibody (S04-3G4) Summary

• Description: Novus Biologicals Rabbit KAT1 Antibody (S04-3G4) (NBP3-19521) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human KAT1 (Uniprot # O14929)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HAT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/200
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for KAT1 Antibody (S04-3G4)

• EC 2.3.1.48
• histone acetyltransferase 1
• histone acetyltransferase type B catalytic subunit
• KAT1

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAT1