Goat anti-Human Kappa Light Chain Secondary Antibody [HRP]

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: LYOPH

Goat anti-Human Kappa Light Chain Secondary Antibody [HRP] Summary

• Description: Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.
  
  Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
• Immunogen: Purified Human Kappa light chain
• Specificity: Based on IEP, this antibody reacts with kappa and light chains on all human immunoglobulins
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGKC
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:5000
• Application Notes: This conjugate is suitable for all immunoassay applications.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins, heavy chains on all human immunoglobulins, and lambda light chains on all human immunoglobulins

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
• Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
• Preservative: 0.1% ProClin 150
• Concentration: LYOPH
• Purity: Affinity purified
• Reconstitution Instructions: Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody [HRP]

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGKC