Kallikrein 5 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 5 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 5
  Ile67-Ser293
  Accession # Q9Y337
• Specificity: Detects human Kallikrein 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse KLK5 is observed and less than 10% cross-reactivity with recombinant human KLK8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 5 Antibody [Unconjugated]

• EC 3.4.21
• EC 3.4.21.4
• Kallikrein 5
• Kallikrein-like protein 2
• kallikrein-related peptidase 5
• KLK5
• KLKL2
• KLK-L2
• KLK-L2EC 3.4.21.-
• SCTE
• SCTEkallikrein-5
• Stratum corneum tryptic enzyme

Background

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).

1. Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
2. Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
3. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
4. Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
5. Kim, H. et al. (2001) Br. J. Cancer 84:643.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 5 Antibody (AF1108)(4)

Publications using AF1108

• Pauline Nauroy, Alexander Nyström Kallikreins: Essential epidermal messengers for regulation of the skin microenvironment during homeostasis, repair and disease Matrix Biology Plus 2020-05-01 [PMID: 33543017]
• Fischer J, Koblyakova Y, Latendorf T, Wu Z, Meyer-Hoffert U Cross-linking of SPINK6 by transglutaminases protects from epidermal proteases. J Invest Dermatol, 2013-01-10;133(5):1170-7. 2013-01-10 [PMID: 23303447] (Bioassay, Human)
• Chia-Yao Lee, David Marzan, Grace Lin, Steve Goodison, Steve Silletti alpha 2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5 Journal of Oncology 2011-12-13 [PMID: 22203845]
• Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma. Biol. Chem., 2007-11-01;388(11):1235-42. 2007-11-01 [PMID: 17976017] (IHC-P, Human)

Bioinformatics

• Gene Symbol: KLK5
• Uniprot:
  • Q9Y337()