Kallikrein 5 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Kallikrein 5 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 5 (R&D Systems, Catalog # 1108-SE)
  Ile67-Ser293
  Accession # Q9Y337
• Specificity: Detects human Kallikrein 5 in Western blots. In this format, less than 1% cross-reactivity with recombinant human Kallikrein 1, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14, and 15 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLK5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 5 Antibody [Biotin]

• EC 3.4.21
• EC 3.4.21.4
• Kallikrein 5
• Kallikrein-like protein 2
• kallikrein-related peptidase 5
• KLK5
• KLKL2
• KLK-L2
• KLK-L2EC 3.4.21.-
• SCTE
• SCTEkallikrein-5
• Stratum corneum tryptic enzyme

Background

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).

1. Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
2. Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
3. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
4. Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
5. Kim, H. et al. (2001) Br. J. Cancer 84:643.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 5 Antibody (BAF1108)(2)

Publications using BAF1108

• Defects in Stratum Corneum Desquamation Are the Predominant Effect of Impaired ABCA12 Function in a Novel Mouse Model of Harlequin Ichthyosis PLoS ONE, 2016-08-23;11(8):e0161465. 2016-08-23 [PMID: 27551807] (ELISA Development (Detection), Human)
• Morizane S, Yamasaki K, Kabigting FD, Gallo RL Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid. J. Invest. Dermatol., 2010-01-21;130(5):1297-306. 2010-01-21 [PMID: 20090765] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: KLK5
• Uniprot:
  • Q9Y337()