Kallikrein 12 Antibody (364932)

The cleavage of Boc-VPR-AMC (0.1 mM, Catalog # ES011) by Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) is measured after preincubation with increasing concentrations of Human Kallikrein 12 Monoclonal Antibody (Catalog # MAB40341). The ND50 is typically 0.1 µg/mL.

• Reactivity: Hu
• Applications: WB, IP, B/N
• Clone: 364932
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 12 Antibody (364932) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 12
  Ala18-Asn248
  Accession # Q9UKR0
• Specificity: Detects human Kallikrein 12 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh) Kallikrein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, rhKallikrein beta 1, rhHGF Activator, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin or rhuPA-1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLK12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 0.1 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 12 Antibody (364932)

• DKFZp686H1078
• EC 3.4.21
• EC 3.4.21.-
• Kallikrein 12
• Kallikrein-like protein 5
• kallikrein-related peptidase 12
• KLK12
• KLK-L5
• KLKL5kallikrein-12
• KLK-L5MGC42603

Background

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family (1). It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat.

1. Yousef, G.M. et al. (2000) Genomics. 69:331.
2. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 12 Antibody (MAB40341)(3)

Publications using MAB40341

• Sato, A;Takagi, K;Yoshimura, A;Tsukamoto, W;Yamaguchi-Tanaka, M;Miki, Y;Ebata, A;Miyashita, M;Suzuki, T; Kallikrein-Related Peptidase 12 (KLK12) in Breast Cancer as a Favorable Prognostic Marker International journal of molecular sciences 2023-05-08 [PMID: 37176127] (IHC, Human)
• Bin L, Kim BE, Hall CF Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin. J. Invest. Dermatol., 2011-07-14;131(11):2213-22. 2011-07-14 [PMID: 21753780] (Neutralization, Human)
• Memari</LastName><ForeNam N</Initial, Memari N, Jiang W, Diamandis EP, Luo LY Enzymatic properties of human kallikrein-related peptidase 12 (KLK12). Biol. Chem., 2007-04-01;388(4):427-35. 2007-04-01 [PMID: 17391064] (Neutralization, Human)

Bioinformatics

• Gene Symbol: KLK12
• Uniprot:
  • Q9UKR0()