Kallikrein 12 Antibody (364926)

• Reactivity: Hu
• Applications: WB
• Clone: 364926
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 12 Antibody (364926) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 12
  Ala18-Asn248
  Accession # Q9UKR0
• Specificity: Detects human Kallikrein 12 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Kallikrein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, rhKallikrein beta 1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin, or rhuPA-1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLK12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 12 Antibody (364926)

• DKFZp686H1078
• EC 3.4.21
• EC 3.4.21.-
• Kallikrein 12
• Kallikrein-like protein 5
• kallikrein-related peptidase 12
• KLK12
• KLK-L5
• KLKL5kallikrein-12
• KLK-L5MGC42603

Background

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family (1). It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat.

1. Yousef, G.M. et al. (2000) Genomics. 69:331.
2. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLK12
• Uniprot:
  • Q9UKR0()