Jade-1/PHF17 Antibody (658005)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Jade‑1/PHF17 Monoclonal Antibody (Catalog # MAB6275) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jade‑1/PHF17 at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Jade-1/PHF17 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Jade-1/PHF17 Monoclonal Antibody (Catalog # MAB6275) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 658005
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Jade-1/PHF17 Antibody (658005) Summary

• Immunogen: E. coli-derived recombinant human JADE-1/PHF-17
  Met1-Ser137
  Accession # Q6IE81
• Specificity: Detects human JADE-1/PHF-17 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human Jade-2/PHF15 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHF17
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jade-1/PHF17 Antibody (658005)

• FLJ22479
• Jade1
• Jade-1
• KIAA1807
• PHD finger protein 17JADE1gene for apoptosis and differentiation in epithelia
• PHD protein Jade-1
• PHF17
• protein Jade-1

Background

Jade-1 (Gene for Apoptosis and Differentiation in Epithelia; also PHF17) is a 94 kDa member of the JADE family of proteins. It is expressed in renal tubule cells as well as embryonic primitive streak (in mouse). Initially, Jade-1 was described as a proapoptotic tumor suppressor. Jade-1 also interacts with the HBO1 complex to specifically acetylate nucleosome histones, thus acting as a transcriptional activator that likely impacts DNA replication, recombination and repair. Human Jade-1 is 842 amino acids (aa) in length. It contains one PEST domain with multiple Ser phosphorylation sites (aa 5-28), and two PHD Zinc fingers (aa 203-253 and 312-371) that are required for histone acetylation. There are two splice variants, one that is 61-64 kDa in size and contains a 7 aa substitution for aa 503-842, and another that shows a deletion of aa 99-110. Over aa 1-137, human Jade-1 shares 96% aa identity with mouse Jade-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHF17
• Uniprot:
  • Q6IE81()