Jade-1/PHF17 Antibody (658005) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 658005
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Jade-1/PHF17 Antibody (658005) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human JADE-1/PHF-17
  Met1-Ser137
  Accession # Q6IE81
• Specificity: Detects human JADE-1/PHF-17 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human Jade-2/PHF15 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jade-1/PHF17 Antibody (658005) [Alexa Fluor® 350]

• FLJ22479
• Jade1
• Jade-1
• KIAA1807
• PHD finger protein 17JADE1gene for apoptosis and differentiation in epithelia
• PHD protein Jade-1
• PHF17
• protein Jade-1

Background

Jade-1 (Gene for Apoptosis and Differentiation in Epithelia; also PHF17) is a 94 kDa member of the JADE family of proteins. It is expressed in renal tubule cells as well as embryonic primitive streak (in mouse). Initially, Jade-1 was described as a proapoptotic tumor suppressor. Jade-1 also interacts with the HBO1 complex to specifically acetylate nucleosome histones, thus acting as a transcriptional activator that likely impacts DNA replication, recombination and repair. Human Jade-1 is 842 amino acids (aa) in length. It contains one PEST domain with multiple Ser phosphorylation sites (aa 5-28), and two PHD Zinc fingers (aa 203-253 and 312-371) that are required for histone acetylation. There are two splice variants, one that is 61-64 kDa in size and contains a 7 aa substitution for aa 503-842, and another that shows a deletion of aa 99-110. Over aa 1-137, human Jade-1 shares 96% aa identity with mouse Jade-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6IE81()