ISG15/UCRP Antibody (3E5) - BSA Free

Western Blot: ISG15/UCRP Antibody (3E5) [NBP1-04310] - Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate

Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (3E5) [NBP1-04310] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ISG15/UCRP Antibody (3E5) - BSA Free Summary

• Immunogen: Recombinant human ISG15/UCRP (1-157aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ISG15
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for ISG15/UCRP Antibody (3E5) - BSA Free

• G1P2
• G1P2interferon-induced 17-kDa/15-kDa protein
• IFI15
• IFI15hUCRP
• interferon, alpha-inducible protein (clone IFI-15K)
• Interferon-induced 15 kDa protein
• Interferon-induced 17 kDa protein
• interferon-stimulated protein, 15 kDa
• IP17
• ISG15 ubiquitin-like modifier
• ISG15
• Ubiquitin cross-reactive protein
• ubiquitin-like protein ISG15
• UCRP
• UCRPIP17

Background

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ISG15/UCRP Antibody (NBP1-04310)(1)

Publication using NBP1-04310

• Zhu G, Badonyi M, Franklin L et al. Type I Interferonopathy due to a Homozygous Loss-of-Inhibitory Function Mutation in STAT2 Journal of clinical immunology 2023-02-08 [PMID: 36753016] (WB, Human)

Bioinformatics

• Gene Symbol: ISG15
• Uniprot:
  • NP_005092(Human)