IRGC Antibody - BSA Free

Western Blot: IRGC Antibody [NBP1-77364] - Mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: IRGC Antibody [NBP1-77364] - A20 cells with IRGC antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: IRGC Antibody - BSA Free [NBP1-77364] - Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IRGC Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC. The immunogen is located within the last 50 amino acids of IRGC. Amino Acid Squence: CLDSWKKHDSEEK
• Specificity: Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRGC
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2.5-20 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  IRGC Peptide (NBP1-77364PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IRGC Antibody - BSA Free

• CINEMA
• EC 3.6.5
• EC 3.6.5.-
• Iigp5
• Immunity-related GTPase cinema 1
• immunity-related GTPase family, cinema 1
• immunity-related GTPase family, cinema
• interferon inducible GTPase 5
• interferon-inducible GTPase 5
• IRGC1
• R30953_1

Background

Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans. Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRGC
• Uniprot:
  • NP_062558(Human)