IRF2 Antibody

Images

 
Western Blot: IRF2 Antibody [NB100-1089] - Staining (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: IRF2 Antibody [NB100-1089] - HEK293 overexpressing IRF2 and probed with (mock transfection in first lane).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IRF2 Antibody Summary

Immunogen
Peptide with sequence KTSDITQARVKSC corresponding to C-Terminus according to NP_002190.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 48 kDa band observed in Jurkat lysates is reported in scientific literature (PMID: 10102295. In transfected HEK293 transiently expressing IRF2 bands of approx. 40 kDa band and 45 kDa band are observed. These bands are not observed in the non-transfected HEK293.
Positive Control
Jurkat Lysate (NB800-PC2)
IRF2 Lysate (NBL1-12031)
Publications
Read Publication using NB100-1089.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRF2 Antibody

  • DKFZp686F0244
  • interferon regulatory factor 2
  • IRF2
  • IRF-2

Background

IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF2 Antibody (NB100-1089)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IRF2 Antibody (NB100-1089) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IRF2 Antibody (NB100-1089)

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Bioinformatics

Gene Symbol IRF2
Entrez
Uniprot