IL-29/IFN-lambda 1 Antibody (247801)

Images

 
Recombinant Human IL‑29/IFN‑ lambda 1 (Catalog # 1598‑IL) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
247801
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

IL-29/IFN-lambda 1 Antibody (247801) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-29/IFN-lambda 1
Gly20-Thr200
Accession # Q8IU54
Specificity
Detects human IL-29/IFN-lambda 1 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) IL-28B/IFN-lambda 3 and 20% with rhIL‑28A is observed. No cross-reactivity or interference was observed with rhIFN-alpha A, rhIFN-alpha B2, rhIFN-alpha G, rhIFN-alpha I, or rhIFN‑ beta 1A.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IFNL1
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 1-4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human IL-29/IFN-lambda 1 Antibody (Catalog # MAB15981)
ELISA Detection: Human IL-29/IFN-lambda 1 Biotinylated Antibody (Catalog # BAF1598)
Standard: Recombinant Human IL-29/IFN-lambda 1 (Catalog # 1598-IL)
Publications
Read Publications using
MAB15981 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-29/IFN-lambda 1 Antibody (247801)

  • cytokine Zcyto21
  • IFNL1
  • IFN-lambda 1
  • IFN-lambda-1
  • IL29
  • IL-29
  • interferon lambda-1
  • interferon, lambda 1
  • interleukin 29 (interferon, lambda 1)
  • interleukin-29
  • ZCYTO21

Background

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11‑13% aa sequence identity) and the type I IFN family (15‑19% aa sequence identity) (1‑3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor  beta (IL-10 R beta ) and a novel IL-28 receptor  alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.

  1. Vilcek, J. (2003) Nature Immunol. 4:8.
  2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-75930
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF874
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, BA, B/N, Flow-IC
NBP1-32905
Species: Hu, Bv, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-24906
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, BA, Flow-IC
6507-IL/CF
Species: Hu
NBP2-35044
Species: Hu
1102-IL/CF
Species: Hu
MAB15041
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
MAB15981
Species: Hu
Applications: B/N, ELISA(Cap)

Publications for IL-29/IFN-lambda 1 Antibody (MAB15981)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Capture, ELISA Development (Capture), Flow Cytometry, Neutralization.


Filter By Application
ELISA Capture
(1)
ELISA Development (Capture)
(1)
Flow Cytometry
(1)
Neutralization
(5)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB15981 Applications Species
V Oke, I Gunnarsson, J Dorschner, S Eketjäll, A Zickert, TB Niewold, E Svenungsso High levels of circulating interferons type I, type II and type III associate with distinct clinical features of active systemic lupus erythematosus Arthritis Res. Ther., 2019;21(1):107. 2019 [PMID: 31036046] (ELISA Capture, Human) ELISA Capture Human
V Oke, S Brauner, A Larsson, J Gustafsson, A Zickert, I Gunnarsson, E Svenungsso IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE) Arthritis Res. Ther., 2017;19(1):139. 2017 [PMID: 28619037] (Human) Human
Iva Gunnarsson Interferon (IFN)-? is a potential mediator in lupus nephritis Lupus Sci Med, 2016;3(1):e000170. 2016 [PMID: 27933198] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Villenave R, Broadbent L, Douglas I, Lyons J, Coyle P, Teng M, Tripp R, Heaney L, Shields M, Power U Induction and Antagonism of Antiviral Responses in Respiratory Syncytial Virus-Infected Pediatric Airway Epithelium. J Virol, 2015;89(24):12309-18. 2015 [PMID: 26423940] (Neutralization, Human) Neutralization Human
Persson B, Jaffe A, Fearns R, Danahay H Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation. PLoS ONE, 2014;9(7):e102368. 2014 [PMID: 25033192] (Neutralization, Human) Neutralization Human
Brownell J, Bruckner J, Wagoner J, Thomas E, Loo Y, Gale M, Liang T, Polyak S Direct, interferon-independent activation of the CXCL10 promoter by NF-kappaB and interferon regulatory factor 3 during hepatitis C virus infection. J Virol, 2014;88(3):1582-90. 2014 [PMID: 24257594] (Neutralization, Human) Neutralization Human
Brownell J, Wagoner J, Lovelace E, Thirstrup D, Mohar I, Smith W, Giugliano S, Li K, Crispe I, Rosen H, Polyak S Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes. J Hepatol, 0;59(4):701-8. 0 [PMID: 23770038] (Neutralization, Human) Neutralization Human
Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells. PLoS Pathog, 2013;9(4):e1003316. 2013 [PMID: 23637605] (Neutralization, Human) Neutralization Human
Yin Z, Dai J, Deng J, Sheikh F, Natalia M, Shih T, Lewis-Antes A, Amrute S, Garrigues U, Doyle S, Donnelly R, Kotenko S, Fitzgerald-Bocarsly P Type III IFNs are produced by and stimulate human plasmacytoid dendritic cells. J Immunol, 2012;189(6):2735-45. 2012 [PMID: 22891284] (Flow Cytometry, Human) Flow Cytometry Human

Reviews for IL-29/IFN-lambda 1 Antibody (MAB15981) (0)

There are no reviews for IL-29/IFN-lambda 1 Antibody (MAB15981). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-29/IFN-lambda 1 Antibody (MAB15981) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NBP2-92973B

Additional IL-29/IFN-lambda 1 Products

Bioinformatics Tool for IL-29/IFN-lambda 1 Antibody (MAB15981)

Discover related pathways, diseases and genes to IL-29/IFN-lambda 1 Antibody (MAB15981). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on IL-29/IFN-lambda 1

There are no specific blogs for IL-29/IFN-lambda 1, but you can read our latest blog posts.
Coronavirus Brochure

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-29/IFN-lambda 1 Antibody (247801) and receive a gift card or discount.

Bioinformatics

Gene Symbol IFNL1
Uniprot