IL-28R alpha/IFN-lambda R1 Antibody (601106) [Unconjugated]

Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-HumanIL‑28 R alpha /IFN‑ lambda R1 Monoclonal Antibody (Catalog # MAB5260, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Peripheral blood mononuclear cells (PBMCs) either unstimulated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human IL-28 Ra/IFN-lambda R1 Monoclonal Antibody (Catalog # MAB5260) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 601106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-28R alpha/IFN-lambda R1 Antibody (601106) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
  Arg21-Ala228
  Accession # Q8IU57
• Specificity: Detects human IL-28 R alpha /IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28R alpha/IFN-lambda R1 Antibody (601106) [Unconjugated]

• class II cytokine receptor CRF2/12
• CRF2/12
• CRF2-12
• Cytokine receptor class-II member 12
• Cytokine receptor family 2 member 12
• IFN-lambda R1
• IFN-lambda receptor 1
• IFN-lambda-R1
• IFNLR
• IFNLR1
• IL-28 R alpha
• IL-28 receptor subunit alpha
• IL28R alpha
• IL-28R1
• IL28RA
• IL-28Ra
• IL-28R-alpha
• Interferon lambda receptor 1
• interferon lambda, receptor 1
• interleukin 28 receptor A
• interleukin 28 receptor, alpha (interferon, lambda receptor)
• interleukin 28 receptor, alpha
• interleukin or cytokine receptor 2
• interleukin-28 receptor subunit alpha
• LICR2
• Likely interleukin or cytokine receptor 2

Background

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta , CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN‑ lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha . Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNLR1
• Uniprot:
  • Q8IU57(Human)