IL-28R alpha/IFN-lambda R1 Antibody (601106) [Phycoerythrin]

Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Peripheral blood mononuclear cells (PBMCs) either untreated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 601106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-28R alpha/IFN-lambda R1 Antibody (601106) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
  Arg21-Ala228
  Accession # Q8IU57
• Specificity: Detects human IL-28 R alpha /IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28R alpha/IFN-lambda R1 Antibody (601106) [Phycoerythrin]

• class II cytokine receptor CRF2/12
• CRF2/12
• CRF2-12
• Cytokine receptor class-II member 12
• Cytokine receptor family 2 member 12
• IFN-lambda R1
• IFN-lambda receptor 1
• IFN-lambda-R1
• IFNLR
• IFNLR1
• IL-28 R alpha
• IL-28 receptor subunit alpha
• IL28R alpha
• IL-28R1
• IL28RA
• IL-28Ra
• IL-28R-alpha
• Interferon lambda receptor 1
• interferon lambda, receptor 1
• interleukin 28 receptor A
• interleukin 28 receptor, alpha (interferon, lambda receptor)
• interleukin 28 receptor, alpha
• interleukin or cytokine receptor 2
• interleukin-28 receptor subunit alpha
• LICR2
• Likely interleukin or cytokine receptor 2

Background

IL-28 R alpha (IL-28 Receptor alpha subunit), also known as Interferon-lambda R1 (IFN-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is Cytokine Receptor Family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta , CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN‑ lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha . Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-28R alpha/IFN-lambda R1 Antibody (FAB5260P)(3)

Publications using FAB5260P

• VM Stone, EE Ringqvist, PG Larsson, E Domsgen, U Holmlund, E Sverremark, M Flodström- Inhibition of Type III Interferon Expression in Intestinal Epithelial Cells-A Strategy Used by Coxsackie B Virus to Evade the Host's Innate Immune Response at the Primary Site of Infection? Microorganisms, 2021-01-05;9(1):. 2021-01-05 [PMID: 33466313] (Flow Cytometry, Human)
• L Wisgrill, I Wessely, A Netzl, L Pummer, K Sadeghi, A Spittler, A Berger, E Förster-Wa Diminished secretion and function of IL-29 is associated with impaired IFN-? response of neonatal plasmacytoid dendritic cells J. Leukoc. Biol., 2019-06-18;0(0):. 2019-06-18 [PMID: 31211458] (Flow Cytometry, Human)
• Y Yu, P Wan, Y Cao, W Zhang, J Chen, L Tan, Y Wang, Z Sun, Q Zhang, Y Wan, Y Zhu, F Liu, K Wu, Y Liu, J Wu Hepatitis B Virus e Antigen Activates the Suppressor of Cytokine Signaling 2 to Repress Interferon Action Sci Rep, 2017-05-11;7(1):1729. 2017-05-11 [PMID: 28496097] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IFNLR1
• Uniprot:
  • Q8IU57(Human)