IL-22R alpha 1 Antibody (305405) [Phycoerythrin]

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 Ra1 PE-conjugated Monoclonal Anti-body (Catalog # FAB2770P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 Ra1 PE-conjugated Monoclonal Anti-body (Catalog # FAB2770P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 305405
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-22R alpha 1 Antibody (305405) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
  Pro18-Thr228
  Accession # Q8N6P7
• Specificity: Detects human IL‑22 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL22RA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22R alpha 1 Antibody (305405) [Phycoerythrin]

• CRF2-9
• CRF2-9interleukin 22 receptor
• Cytokine receptor class-II member 9
• Cytokine receptor family 2 member 9
• IL-22 R alpha 1
• IL-22 receptor subunit alpha-1
• IL22R alpha 1
• IL22R
• IL22R1
• IL22RA1
• IL-22Ra1
• IL-22R-alpha-1
• IL-TIF-R1
• interleukin 22 receptor, alpha 1
• interleukin-22 receptor subunit alpha-1
• zcytoR11

Background

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
13. Wolk, K. et al. (2004) Immunity 21:241.
14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-22R alpha 1 Antibody (FAB2770P)(4)

Publications using FAB2770P

• Nicholas J. Protopsaltis, Wei Liang, Eric Nudleman, Napoleone Ferrara Interleukin-22 promotes tumor angiogenesis Angiogenesis 2019-05-01 [PMID: 30539314]
• Fangchen Gong, Jin Wu, Ping Zhou, Mengyao Zhang, Jingning Liu, Ying Liu et al. Interleukin-22 Might Act as a Double-Edged Sword in Type 2 Diabetes and Coronary Artery Disease Mediators of Inflammation 2016-10-18 [PMID: 27829708] (Flow Cytometry)
• Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH TH17 cytokines induce human airway smooth muscle cell migration. J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2. 2011-02-22 [PMID: 21345484] (Flow Cytometry, Human)
• Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009-10-01;39(10):2779-88. 2009-10-01 [PMID: 19731362] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL22RA1
• Uniprot:
  • Q8N6P7()