IL-10 R beta Antibody (90220) [Allophycocyanin]

• Applications: Flow
• Clone: 90220
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

IL-10 R beta Antibody (90220) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
  Met20-Ser220
  Accession # Q08334
• Specificity: Detects human IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑10 R alpha , rhIFN-gamma RI, and rhIFN-gamma R2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL10RB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

IL-10 mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4^th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL-10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously. IL-10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
5. Lutfalla, G. et al. (1993) Genomics 16:366.
6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL10RB