Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody

Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.

ELISA: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - ELISA Results of Rabbit anti-Mouse IgM Heavy Chain Secondary antibody tested against purified Mouse IgM. Each well was coated in duplicate with 10 ug of Mouse IgM. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Rabbit IgG mx10 HRP conjugated, and TMB substrate.

• Reactivity: Mu
• Applications: WB, DB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgM and Mouse Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Mouse IgM whole molecule
• Specificity: No reaction was observed against other mouse heavy or light chain proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:500 - 1:2500
  • Western Blot 1:2000 - 1:10000
• Application Notes: This product has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM